The sixth-generation American and seven-time Wimbledon winner was clearly in pain at the slippery court and sought treatment 3-2 in his first match against Belarusian Aliaksandra Sasnovich.
Williams returned after a long break but grief was evident.She was shocked and wiped away the tears before preparing to serve 3-3 after Sasnovich bounced back from a 3-1 down.
The 39-year-old, who started the game by tying his right thigh, screamed and knelt down and cried, before being helped in court.
Tennis player Serena Williams has won more Grand Slam titles than any other woman or man in the competition. In addition, Serena and her sister Venus won double Grand Slam titles, second in the open season, and three double gold medals at the Olympics.
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